Green biosynthises of nanosilver using Gylcyrrhiza glabra extract -screening of its antioxidant, antimicrobial and antidiabetic activity

Abstract

The current study aims to investigate the anti-bacterial, anti-fungal, antidiabetic and anti-oxidant properties of the silver nanoparticles synthesised by ecofriendly method of green biosynthesis by utilizing the aqueous stem extract of Glycyrrhiza glabra (Licorice Stem) as a reducing agent. The bio synthesised AgNPs are characterised in this study using visual colour change, FTIR study was used to detect the presence of functionals groups present in the AgNP, SEM analysis was used to characterise the average size of AgNPs, which were with a size range from 64.82 nm to 85.04 nm and UV-Vis spectroscopic analysis shows maximum absorbance band at 450 nm, respectively. Using the agar disc diffusion method, antibacterial and antifungal activity was evaluated against typical strains of bacteria and fungus. The antioxidant property was determined using DPPH assay and SOD assays. The antidiabetic property was tested using alpha amylase and alpha glucosidase inhibitory assays respectively and the results were compared with the results of acarbose standards. Due to its high concentration of plant-based phytoestrogens and/or natural antioxidants, the legume Glycyrrhiza glabra may be used in the long run to treat and/or prevent diseases linked to oxidative stress including diabetic nephropathy, ferroptosis and certain types of cancer and nanobiomedial applications.